ABSTRACT
Objective To establish a method for the quality control of Qingyan Dropping Pill.MethodsAn HPLC method was developed to establish the fingerprint of polar components in Qingyan Dropping Pill,and 12 samples from various batches were analyzed.Furthermore,principal component analysis(PCA) was used to differentiate and evaluate the whole fingerprints.The relationship between Qingyan Dropping Pill and their original herbs were investigated with HPLC-DAD method.Results The result showed that liquiritin was the key component of quality control.Among the chromatographic peaks,there were six chromatographic peaks coming from Glycyrrhizae Radix et Rhizoma;Five chromatographic peaks coming from Chebulae Fructus.Conclusion This method can be used as quality control for Qingyan Dropping Pill.
ABSTRACT
Objective To study the separation and purification technology of iridoid glycosides and xanthones from Swertia mussotii by macroporous resin.Methods The dynamic adsorption and desorption characteristics of macroporous adsorption resins HPD-300,HPD-400,HPD-600,AB-8,DM-301,and D-101-Ⅰ for swertiamarin and swertianolin were investigated.On the base of the investigation,the better macroporous adsorption resin was chosen to finish the experiments.Results The adsorption and elution characteristics of HPD-300 used to separate and purify iridoid glycosides and xanthones were very good.In the course of adsorption,the amount of used adsorption is 0.9 g dried medicinal herb/mL resin,with about 8 pH value of adsorption solution,and the volumetric rate is 2 BV/h; In the course of elution,the resin column chromatography was eluted gradiently with 7 BV 20% and 5 BV 70% EtOH after removing impurities with water.The elution rates were both more than 90%,and the contents of iridoid glycosides and xanthones were both up to 50% in the two dried fractions of 20% and 70% ethanolic elutions.Conclusion HPD-300 is an ideal resin with the best enrichment for separating and purifying both iridoid glucosioles and xanthanoes simultanously.